Use of cyanoacrylate-based surgical adhesives associated to the macroporous tape in skin synthesis in rats

Abstract Purpose: To compare the use of new cyanoacrylate surgical adhesive associated with macroporous tapes in cutaneous synthesis. Methods: Male Wistar rats with a longitudinal incision of 4cm were used on the back, divided into four groups: GI used octyl-cyanoacrylate (Dermabond®), GII used N-2-butylcyanoacrylate, GIII used octyl-cyanoacrylate and macroporous tape and GIV used N-2-butyl cyanoacrylate and macroporous tape. On the fourteenth day, the rats were submitted to euthanasia, were divided in two parts, and a layer of skin subcutaneous tissue through an area of operative healing was removed. One part was submitted to the study of rupture strength with the use of tensiometer, and in the other part histological examination was performed. Results: No force test was similar between groups I and II, being different from groups III and IV (P <0.001), which were identical to each other (P> 0.05). The units were compared among the studied groups, and they were different with the use of macroporous tapes (P> 0.05). Conclusions: The purpose of macroporous tapes is associated with CA adhesives in cutaneous tissues that provide more resistant scars. The use of a combination of macroporous tapes leads to complete re-epithelialization, without provoking foreign body reaction, has hemostatic properties and does not cause an absorptive reaction.

Efeito de cianoacrilatos e adesivo tissular a base de veneno de cobra na citotoxicidade sobre fibroblastos gengivais e estresse oxidativo sobre células do biofilme de C. albicans / Effect cyanoacrylate and snake venom tissue adhesive on cytotoxicity of gingival fibroblasts and oxidative stress of C. albicans biofilms

A estomatite protética é uma patologia que acomete muitos usuários de prótese total. Um dos principais fatores da sua etiologia é a fácil adesão de microrganismos, como a Candida albicans, à resina acrílica, principalmente devido às características de superfície facilitadoras deste material. Por esta razão, estuda-se materiais que possam modificar as características de superfície da resina acrílica sem alterar as suas propriedades mecânicas. A investigação das propriedades desses materiais, como a biocompatibilidade e como eles influenciam as células de C. albicans, é de extrema importância para uma futura aplicação clínica. Dessa forma, a proposta desta pesquisa foi avaliar a citotoxicidade sobre fibroblastos gengivais humanos (FGH) e o estresse oxidativo causado em células de C. albicans por 6 produtos (etil-cianoacrilato convencional -ECAc; etil-cianoacrilato em forma de gel formulação a -ECAga; etil-cianoacrilato em forma de gel formulação b -ECAgb; butil-cianoacrilato - BCA; octil-cianoacrilato OCA; selante tissular a base de veneno de cobra-VC;), que quando aplicados na superfície da resina acrílica, modificam suas características de superfície, visando diminuir a formação do biofilme de C. albicans. Espécimes de resina acrílica foram confeccionados e duas camadas de cada produto foram aplicadas nas superfícies. Cada espécime foi deixado em 1, 066 mL de meio de cultura durante 24 horas para extração e utilização do meio condicionado para os dois ensaios. Para a investigação da citotoxicidade, os FGH foram cultivados em placas de 96 poços, meios condicionado de cada grupo foram depositados em cada poço onde permaneceram em contato por 24, 48 e 72 h. Após o processamento para o ensaio de MTT as placas foram analisadas em espectrofotômetro a 500 nm. Foram realizados quatro experimentos independentes em triplicata (n=12). Para a investigação de estresse oxidativo (ERO) causado em C. albicans, biofilme foi desenvolvido em placas de 96 poços durante 24 h. Então, os meios condicionados foram adicionados aos poços e a placa foi mantida em agitadora durante 1 h. Após a retirada dos meios condicionados, foi adicionado a cada poço o reagente CellRox® Deep Red e leituras foram realizadas a cada 30 min durante 3 h em fluorímetro a 640/665nm. Amostras foram coletadas e ensaio de cultura microbiológica foi executado para a realização da normalização dos valores. Foram realizados 3 experimentos independentes em triplicata (n=9). Os resultados obtidos pelo ensaio MTT foram analisados por meio de ANOVA a 2 critérios, seguido pelo teste Tukey para a comparação entre grupos (p<0,05). Para o ensaio ROS, a análise estatística realizada foi ANOVA a 1 critério, seguida, igualmente, por Tukey (p<0,05). Os resultados mostraram que todos os grupos apresentaram certo grau de citotoxicidade quando comparados ao controle, com exceção do VC (ECAc

Denture stomatitis is a disease that affects many denture wearers. One of the main factors of its cause is the easy adhesion of microorganisms, such as Candida albicans, to acrylic resin, mainly due to its permissive surface characteristics. For this reason, materials that can modify surface characteristics of acrylic resin without changing its mechanical properties are being studied. The investigation of these materials features, such as biocompatibility and how they affect Candida albicans, is of utmost importance for future clinical application. Thus, the purpose of this study was to evaluate the cytotoxicity of human gingival fibroblasts (HGF) and oxidative stress in C. albicans cells after contact with 6 experimental products (conventional ethyl-cyanoacrylate -ECAc; ethyl-cyanoacrylate in gel formulation "a" -ECAga; ethyl-cyanoacrylate in gel formulation "b" -ECAgb; butyl- cyanoacrylate - BCA; octyl-cyanoacrylate -OCA; tissue adhesive based on snake venom -VC) applied to the surface of the resin acrylic, modifying its surface characteristics, in order to decrease C. albicans biofilm formation. Acrylic resin specimens were manufactured and two layers of each product were applied to the surfaces. Each specimen was immersed in 1,066 mL of growth media for 24 hours for extraction and the resulting conditioned media were used for both tests. To investigate cytotoxicity, HGF were cultured in 96-well plates and conditioned media from each group were deposited into each well, where they remained in contact for 24, 48 and 72h. After the MTT assay processing, plates were analyzed in a spectrophotometer at 500 nm. Four independent experiments were performed in triplicate (n=12). To investigate oxidative stress (ROS), C. albicans biofilm was developed in 96-well plates for 24 h, conditioned media were added to the wells and the plate was allowed to stir for 1 h. Then, conditioned media were removed and CellRox® Deep Red reagent was added to each well. Readings were performed every 30 min for 3 h in fluorometer at 640/665nm. Samples were collected and microbiological culture test was executed to values normalization. Three independent experiments were performed in triplicate (n=9). Results obtained in MTT assay were analyzed by two-way ANOVA and Tukey's test for comparison between groups (p<0.05). for comparison between groups (p<0.05). For ROS results, one-way ANOVA was performed followed by Tukey test, as well (p<0.05). The results showed that all groups presented different degrees of cytotoxicity when compared to control group, except for VC (ECAc

Influência do selante de fibrina na cicatrização das pregas vocais de suínos / Influence of selant fibrin on the wound healing of the pigs vocal folds

Os selantes de fibrina ou cola de fibrina são produtos originários de proteínas do plasma humano que mimetizam a via final da rede de coagulação. Sua aplicação para estimular a cicatrização tem sido motivo de discussão na literatura mundial. O uso do selante de fibrina em fonocirurgia tem sido realizado de modo empírico. Não há trabalhos que investiguem a ação do selante de fibrina no espaço de Reinke. OBJETIVOS: Avaliar a interferência do uso da cola de fibrina no processo de cicatrização gerado pela manipulação cirúrgica em pregas vocais de suínos. MATERIAIS E MÉTODOS: Estudo prospectivo e experimental. Seis animais tiveram ambas as pregas vocais incisadas e em apenas uma delas foi aplicado o selante, sendo que a outra serviu de controle. Após 3 meses, os animais foram sacrificados e a contagem de colágenos realizada. RESULTADOS: O lado com aplicação de cola teve média de 27,8 por cento contra 20,4 por cento do lado sem aplicação de cola. CONCLUSÃO: A concentração de colágeno nas amostras onde o selante de fibrina foi aplicado é significativamente maior do que nas amostras onde não houve a aplicação. Portanto, a presença do selante de fibrina estimula a fibrogênese neste tecido.

Fibrin sealants or fibrin glue are products made from human plasma proteins, which mimic the final pathway of the coagulation cascade. Its application to stimulate the healing process has been a topic of debate in the literature. The use of fibrin sealants in phonosurgery has been empirical; there have been no studies that investigate the action of fibrin sealant in Reinke's space. AIM: To evaluate the effect of fibrin glue in healing of the vocal folds of pigs after surgical manipulation. MATERIALS AND METHODS: This was a prospective and experimental study. Six animals had both vocal folds incised. Sealant was applied in one of them; the other served as a control. After three months, the animals were sacrificed and a collagen count was carried out. RESULTS: The side on which glue was applied had an average of 27.8 percent against 20.4 percent of the side without glue. CONCLUSION: The collagen concentration in the samples where the fibrin sealant was applied was significantly higher compared to samples without glue. Thus, the presence of a fibrin sealant stimulates fibrogenesis in this tissue.

A Comparative Study of the Effect of Fibrin Glue versus Sutures on Clinical Outcome in Patients Undergoing Pterygium Excision and Conjunctival Autografts

PURPOSE: To compare the effect of using fibrin glue or 10-0 nylon sutures on the clinical outcome of patients undergoing pterygium excision and conjunctival autografting. METHODS: We retrospectively reviewed the medical records of 52 eyes from 46 patients who underwent pterygium excision and conjunctival autografting and were followed up for more than 3 months. The operation duration, postoperative inflammation, complications, and recurrence rates were compared between groups of 20 patients (22 eyes) for whom fibrin glue was used (fibrin glue group) and 26 patients (30 eyes) for whom suturing was performed with 10-0 nylon (suture group) in pterygium excision and conjunctival autografting. RESULTS: The operation duration was 27.71 (5.22) minutes in the fibrin glue group and 43.30 (8.18) minutes in the suture group (p = 0.000). Seven days after the operation, the fibrin glue group showed milder conjunctival inflammation than the suture group (p = 0.000). Postoperative complications and corneal recurrence rates were not statistically different between the two groups. CONCLUSIONS: The use of fibrin glue in pterygium excision with conjunctival autografting is likely to be a more effective, safer procedure than suturing.

A Comparison of 2-Octyl Cyanoacrylate Adhesives versus Conventional Suture Materials for Eyelid Wound Closure in Rabbits

PURPOSE: To evaluate the clinical efficacy and histopathological tolerance of 2-octyl cyanoacrylate versus conventional suture materials for eyelid wound closure in rabbits. METHODS: We performed an experimental study on 16 eyes of eight New Zealand albino rabbits. Eyelid incisions of 15 mm were done 4mm from the upper eyelid margin in both eyes. The eyes of the rabbits were divided into two groups: eyelid incisions of the right eye were closed by a 2-octyl cyanoacrylate adhesive (group A) and eyelid incisions of the left eye were closed by 7-0 nylon sutures (group B). At 1, 2, 4, and 8 weeks after surgery, the rabbits were macroscopically examined and then sacrificed. The specimens of their eyelid tissues were stained by a hematoxylin and eosin stain and Masson-trichrome stain, and were observed under microscope. RESULTS: Both eyelid surgical closure methods were found to be equally efficacious in fixing the eyelids of groups A and B, and their clinical efficacy was similar. Histopathological findings of the hematoxylin and eosin stain of group A showed less inflammatory infiltration than group B at 2 weeks. There were no significant histopathological differences between the two groups at 1, 4, and 8 weeks. The degree of fibrosis of the Masson-trichrome stain was similar between the two groups at 8 weeks. CONCLUSIONS: The 2-octyl cyanoacrylate adhesive proved to be an effective eyelid closure method and was very well tolerated by the skin surface. 2-Octyl cyanoacrylate could be used as an alternative tissue adhesive for eyelid wound closure along with conventional suture materials.

Fibrin glue in ophthalmology.

Suturing is a time consuming task in ophthalmology and suture induced irritation and redness are frequent problems. Postoperative wound infection and corneal graft rejection are examples of possible suture related complications. To prevent these complications, ophthalmic surgeons are switching to sutureless surgery. A number of recent developments have established tissue adhesives like cyanoacrylate glue and fibrin glue as attractive alternatives to sutures. A possible and promising new application for tissue adhesives is to provide a platform for tissue engineering. Currently, tissue glue is being used for conjunctival closure following pterygium and strabismus surgery, forniceal reconstruction surgery, amniotic membrane transplantation, lamellar corneal grafting, closure of corneal perforations and descematoceles, management of conjunctival wound leaks after trabeculectomy, lid surgery, adnexal surgery and as a hemostat to minimise bleeding. The purpose of this review is to discuss the currently available information on fibrin glue.

Antibacterial properties of cyanoacrylate tissue adhesive: Does the polymerization reaction play a role.

Purpose: To ascertain if the polymerization reaction also contributes additionally to the antibacterial effects of two commonly used cyanoacrylate tissue adhesives. Materials and Methods: Fresh liquid ethyl-cyanoacrylate (EC) and N-butyl-cyanoacrylate (BC) adhesives were applied onto 6-mm sterile filter paper discs. In the first group, the adhesive-soaked discs were immediately placed onto confluent monolayer cultures of bacteria, allowing the polymerization reaction to proceed while in culture. In the second group, the adhesive-soaked disc was allowed to first polymerize prior to being placed onto the bacterial cultures. Four types of bacteria were studied: Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, and Pseudomonas aeruginosa. Immediately after the discs were applied, the cultures were incubated at 35° C for 24 h. Bacterial inhibitory halos were measured in the cultures at the end of the incubation period. Results: For EC, exposure of the bacteria to the cyanoacrylate polymerization reaction increased the bacterial inhibitory halos in Streptococcus pneumonia, Staphylococcus aureus and Escherichia coli. For BC, it increased the bacterial inhibitory halos in Staphylococcus aureus and Streptococcus pneumoniae. No inhibitory halos were observed in Pseudomonas aeruginosa. The bactericidal effect was higher in actively polymerizing EC, compared to previously polymerized EC in Staphylococcus aureus, Streptococcus pneumoniae, and Escherichia coli; however, no such differences were observed for BC. Conclusions: The polymerization reaction may also be an important factor in the antibacterial properties of EC and BC.

Fibrin adhesive and the vaginal vault synthesis on female rabbits abdominal hysterectomies / Adesivo de fibrina e a síntese da cúpula vaginal após histerectomia abdominal em coelhos fêmeas

PURPOSE: To investigate the effectiveness of fibrin glue in comparison with polyglycolic acid suture to promote the closure of rabbit's vaginal vault, after abdominal hysterectomy. METHODS: Twenty female, adults, New Zealand rabbits, were submitted to abdominal hysterectomy and randomly distributed to polyglycolic acid suture (G-PA / n=10) or fibrin glue closure of vaginal vault (G-FG / n=10). Radiograph study allowed identifying vault vaginal suture disrupter or fistulas to urinary bladder or rectum. Videovaginoscopy study allowed identifying the presence of cellulites, abscess formation, tissue granulation or granuloma. Vaginal cuff burst test allowed to identify by the escape of air bubbles and rupture pression record. Histological sections stained with Picrosirius red allowed the measure of fibrous tissue healing. RESULTS: The videovaginoscopy identified a significant difference (Fisher Test p<0.3142) of granulation tissue in the animals of G-PA (40 percent) in comparison with the G-FG (20 percent). The gross inspection showed the same relation in the granulation tissue occurrence (Fisher test p< 0.1749) with G-PA (50 percent) and G-FB (20 percent). The visceral adhesion to the vaginal vault wound was statistical significant (Fisher test p< 0.1749) with G-PA (50 percent) and G-FG (20 percent). The pressure of rupture (mm Hg) of the burst test was similar (p<0.0421) in the animals of G-PA (61.5±19.3) and G-FG (72.5±21.9). The collagen matrix of vault wound healing was similar (p< 0.0231) between the G-PA (31.63±15) and the G-FG (23.2±13.2). CONCLUSION: The vaginal vault closure using the fibrin glue is a safe and reliable procedure after abdominal hysterectomy in female rabbit model.

OBJETIVO: Investigar a eficácia da cola de fibrina e da sutura usando fio de ácido poliglicólico para promover o fechamento da cúpula vaginal de coelhas, após histerectomia abdominal. MÉTODOS: Vinte coelhos fêmeas, New Zealand, adultas foram submetidas à histerectomia abdominal e distribuídas para sutura da cúpula vaginal com fio de ácido poliglicólico (G-PA / n=10) ou cola de fibrina (G-FG / n=10). Estudo radiológico foi realizado para identificar deiscências ou fístulas vesicais ou retais. Videovaginoscopia foi realizada para identificar a presença de secreções, abscessos, tecido de granulação ou granuloma tipo corpo estranho. Teste in vitro de pressão de rompimento sob selo d'água foi realizado para identificar a perviedade da sutura. O tecido de cicatrização foi estudado pela coloração com picrosirius red para mensuração do tecido fibrótico. RESULTADOS: A videovaginoscopia mostrou uma diferença significante (Teste de Fisher p<0,3142) no tecido de granulação do grupo G-PA (40 por cento) em comparação com o grupo G-FG (20 por cento). A análise macroscópica mostrou a mesma relação com o tecido de granulação (Teste de Fisher p< 0.1749) com G-PA (50 por cento) e G-FB (20 por cento). A aderência visceral à cúpula vaginal foi estatisticamente significativa (Teste de Fisher p< 0.1749) com o G-PA (50 por cento) e G-FG (20 por cento). A pressão de rompimento (mmHg) foi similar (p<0.0421) nos animais do grupo G-PA (61.5±19.3) e G-FG (72.5±21.9). A matriz colagenosa do tecido de cicatrização teve resultados semelhantes (p< 0.0231) entre G-PA (31.63±15) e G-FG (23.2±13.2). CONCLUSÃO: O fechamento da cúpula vaginal usando cola de fibrina é um procedimento seguro e confiável após histerectomia abdominal em coelhos fêmeas.

In vitro antimicrobial efficiency of a mouthwash containing triclosan/gantrez and sodium bicarbonate

Several antiseptic substances have been used as adjuncts to routine mechanical procedures of oral hygiene, based on their antimicrobial effects. The objective of this study was to assess in vitro the antimicrobial efficiency of a mouthwash containing Triclosan/Gantrez and sodium bicarbonate in comparison to both positive and negative controls. Standard strain samples of Escherichia coli, Pseudomonas aeruginosa, Actinomyces viscosus and Bacillus subtilis were used. Samples of Streptococcus mutans and Gram-negative bacilli were collected from 20 volunteers (10 with a clinically healthy periodontium and 10 presenting biofilm-associated gingivitis). Evaluation of the antimicrobial activity was performed by determining the minimal inhibitory concentration (MIC). The results indicated that the test solution inhibited the growth of both Gram-negative and Gram-positive microorganisms from the volunteers’ saliva as well as that of the standard strains at the MIC dilution of 1:20, whereas the MIC dilution of 0.12 percent chlorhexidine against the same bacteria was 1:80. Thus, even though the tested mouthrinse solution presented an in-vitro antimicrobial activity superior to that of a placebo, it was inferior to that of chlorhexidine.

Diversas substâncias antisépticas têm sido utilizadas como adjuntos aos procedimentos mecânicos rotineiros de higiene oral, com base em seus efeitos antimicrobianos. O objetivo deste estudo foi avaliar, in vitro, a eficiência antimicrobiana de um enxaguatório bucal contendo Triclosan/Gantrez e bicarbonato de sódio, em comparação a controles positivos e negativos. Linhagens padrão de Escherichia coli, Pseudomonas aeruginosa, Actinomyces viscosus e Bacillus subtilis foram utilizadas. Amostras de Streptococcus mutans e Bacilos Gram-negativos foram coletadas de 20 voluntários (10 com um periodonto clinicamente saudável e 10 com gengivite associada à presença de biofilme). A avaliação da atividade antimicrobiana foi realizada pela determinação da Concentração Inibitória Mínima (CIM). Os resultados mostraram que a solução teste inibiu o crescimento de microrganismos Gram-negativos e Gram-positivos da saliva dos voluntários, bem como das linhagens padrão na CIM de 1:20, enquanto que a CIM da diluição de clorexidina 0.12 por cento contra as mesmas bactérias foi de 1:80. Desta forma, apesar de o enxaguatório bucal testado apresentar atividade antimicrobiana in vitro superior à do placebo, esta foi inferior à da Clorexidina.

The role of biological adhesive and suture material on rabbit hepatic injury / O papel da cola biológica e do fio de sutura na lesão hepática em coelhos

PURPOSE: To evaluate the performance of fibrin adhesive and absorbable suture thread in the repairing of hepatic injures in rabbits. METHODS: New Zealand albino rabbits (n=16), males and females, from 5 to 6 months old, average weight of 2500 g, were distributed randomly in Group A (n-8) - biological adhesive and Group B (n=8) - suture thread. After anesthesia with acepromazine (1mg/Kg), ketamine (50mg/Kg) and fentanyl EV (0,5ml/Kg), it was performed a supra-umbilical median laparotomy, the median hepatic lobe was isolated and subjected to severe standardized incision. In the group B the incision edges were sutured with simple 4-0 catgut, in separated stitches. It was evaluated the total time of the procedure, the hemostasis time and hemorrhage volume. In the 21st post-operative day it was evaluated the presence of adherences and signs of infection in the abdominal cavity, and it was followed by the resection of the median hepatic lobe for the histological evaluation. RESULTS: The calculated mean and standard deviation showed that the procedure time, hemostasis time and bleeding amount were significantly smaller in the group of animals subjected to the use of fibrin adhesive. The surgical abdominal incision was significantly more extensive in the animals of the suture group (average of 6,8 cm) in relation to the adhesive group (average of 3,8), as well as the number of occurrences of abscesses. The adherence of the intestinal ansas to the sutured incision (group B) occurred in five cases and the major omentum adhesion occurred in all animals. In the group A (adhesive) it occurred adherences of the major omentum in three cases. The microscopy of the hepatic incision repaired with the use of fibrin showed that the inflammatory infection is less intense, not associated with the formulation of secretion in the abscesses, and therefore has a more favorable later cicatricial aspect than a conventional suture with surgical thread. CONCLUSION: In agreement with other...

OBJETIVO: Avaliar o desempenho do adesivo de fibrina e do fio de sutura absorvível no reparo de lesões hepáticas provocadas em coelhos. MÉTODOS: Coelhos (n=16),Nova Zelândia, albinos, machos e fêmeas, 5 a 6 meses de idade, peso médio de 2500 gramas, foram distribuídos aleatoriamente em Grupo A(n = 8) - adesivo biológico e Grupo B(n=8) - fio de sutura. Após anestesia com acepromazina (1mg/Kg), quetamina (50mg/Kg) e fentanylEV (0,5ml/Kg), realizou-se laparotomia mediana supra-umbilical, o lobo hepático médio foi isolado e submetido a uma lesão padronizada grave. No grupo A foi aplicado o adesivo de fibrina. No grupo B as bordas da lesão foram suturadas com categute 4-0 simples, em pontos separados. Avaliou-se o tempo total de procedimento, tempo de hemostasia e volume da hemorragia. No 21° dia de pós-operatório avaliou-se a presença de aderências e sinais de infecção na cavidade e procedeu-se a ressecção do lobo hepático médio para a avaliação histológica. RESULTADOS: A média e desvio-padrão calculados mostraram que o tempo de procedimento, tempo de hemostasia e quantidade de sangramento foram significantes menores no grupo de animais submetidos ao uso do adesivo de fibrina. A extensão da ferida operatória foi significativamente maior nos animais do grupo sutura (média de 6,8cm) em relação ao grupo adesivo (média de 3,8cm) assim como o número de vezes da ocorrência de abscessos. A aderência de alças intestinais à lesão suturada (grupo B) ocorreu em 5 casos e adesão do omento maior ocorreu em todos os animais. No grupo A (adesivo) foram observadas aderências do omento maior em três casos. A microscopia da lesão hepática reparada com o uso de fibrina mostrou que a reação inflamatória é menos intensa, não está associada à formação de secreção ou abscessos e com isso tem um aspecto cicatricial tardio mais favorável que uma sutura convencional com fio cirúrgico. CONCLUSÃO: Em concordância com outros trabalhos da literatura biomédica, o adesivo de fibrina é uma opção...

Effects of Tissucol™ and epsilon aminocaproic acid in the healing process following dental extraction in dehydrated rats

Foi estudada histologicamente a reparação do alvéolo dental de ratos desidratados, após o implante de adesivo fibrínico (TISSUCOL®) associado à irrigação prévia da ferida com solução a 5% de ácido épsilon-aminocapróico. Foram empregados 72 ratos, divididos em três grupos, que receberam diferentes tratamentos após o procedimento cirúrgico. No Grupo I, após a extração do incisivo superior direito, a mucosa gengival foi suturada. Nos Grupos II e III foi provocada a desidratação crônica pela privação de ingestão de líquidos durante 09 dias (3 dias no pré-operatório e 6 dias no pós-operatório), sendo que, no Grupo II, após a extração dental, a mucosa gengival foi suturada de forma semelhante à do Grupo I; no Grupo III, logo após a exodontia, o alvéolo foi irrigado com a solução de ácido épsilon-aminocapróico a 5%, seguida de implante de adesivo fibrínico (TISSUCOL®) e foi feita sutura da mucosa de forma semelhante à dos demais grupos. Decorridos 3, 7, 15 e 21 dias após o ato operatório, os animais foram sacrificados em número de 6 para cada grupo. A seguir, as peças contendo o alvéolo dental foram removidas e fixadas em formalina a 10% e descalcificadas em solução de ácido fórmico e citrato de sódio em partes iguais. Após processamento laboratorial de rotina, foram incluídas em parafina para microtomia. Foram obtidos cortes semi-seriados com 6 micrômetros de espessura, seguidos de coloração em hematoxilina e eosina para estudo microscópico. Os resultados obtidos mostram que a hidroprivação no pré e pós-operatório ocasiona profundo retardo no processo de reparo em feridas de extração dental. O emprego de adesivo fibrínico (TISSUCOL®) melhora o quadro fibrinolítico provocado pela desidratação.

The use of snake venom derived fibrin glue in hysterorrhaphy of ovine caesarean surgery

Fibrin glue has been used on its own or in conjunction with suturing materials to promote hemostasis, reduce adherence, strengthen the wound site, and improve healing. Snake venom derived fibrin glue was evaluated as an alternative to conventional uterine suturing after ovine caesarean surgey. Twenty-eight pregnant ewes of known mating date were used. The animals submitted to conventional caesaream sections showed a better wound healing process. As expected, all the operated animals had retained placenta, compromissing coaptation of wound edges. This had a strong influence in the results observed with the animals in which fibrin glue was used. The animals were divided into four groups GI, GII, GIII, and GIV and sacrificed, respectively, 3, 7, 15, and 30 days after surgery for macro and microscopic examination of the uterus. From each group, six animals underwent surgery using fibrin glue and four animals were submitted to comparative conventional hysterorrhaphy using catgut.

Effects of tissue adhesive (Tisseel(R)) on corneal wound healing in lamellar keratoplasty in rabbits

The purpose of this study was to compare corneal wound healing between suture and sutureless lamellar keratoplasty in the rabbit eye. The tissue adhesive (Tisseel, Vienna) a commercially available two component adhesive system based on human fibrinogen, which is activated by thrombin, was used. 8.0mm half-thickness lamellar keratoplasties (autotransplants) were performed on the twenty-four rabbit eyes with tissue adhesive and the other eyes were operated with 10-0 nylon continuous suture as a control, respectively. At varying periods postoperatively, they were killed and the eyes were immediately enucleated and examined by transmission electron microscopy and light microscopy. The procedure was completed by the application of a bandage soft contact lens. Seventy-one percent of the glued lamellar keratoplasty grafts were retained and in six eyes, focal white opacities at the bed and graft interface were noted. There was no change in corneal contour, with the only irregularity being at the graft/host junction in the tissue adhesive group. Histopathologic examination of the glued eyes demonstrated the presence of a layer of eosinophilic material between the corneal bed and the lamellar graft but a minimal inflammatory response was observed in the early postoperative days. At fourteen days the adhesive was no longer visible and at three weeks, the graft was well healed. We believe that this study indicates a potential adjunctive role for tissue adhesive in corneal wound healing.